Fig 9. The constitutive expression of GhWRKY68 increased ROS production and oxidative damage in transgenic plants.

(A) Representative photographs of H₂O₂ accumulation using DAB staining. For drought and salinity treatments, the top figures show H₂O₂ accumulation, and the bottom figures illustrate the microscopic observations of the brown precipitate. (B) Phenotypes of young seedlings grown on medium containing 2 μM MV. (C) Quantification of cotyledon greening in the seedlings described in (B). The data represent the means ± SE of three independent experiments. The different letters above the columns in (C) indicate significant differences (P < 0.05) according to Duncan’s multiple range test performed using SAS version 9.1 software.