Table 2. Kinetic parameters for reconstituted human RISC (37°C).

dsRNA substrate	Purified recombinant RISC proteins	K m,app (nM)	k cat (s-1)	Substrate Inhibition, K i (nM)	k cat / K m,app (M-1 s-1)
BoGD664	DICER	122	2.88×10-4	-	2360
	DICER+AGO2	139	5.31×10-4	-	3820
	DICER+TRBP	77	2.14×10-4	-	2770
BoPD664	DICER	165	3.11×10-4	-	1890
	DICER+AGO2	359	7.68×10-4	-	2140
BoPsi664	DICER	19	6.69×10-6	-	344
	DICER+AGO2	74	7.28×10-5	105	979
	DICER+TRBP	85	1.16×10-5	-	137