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. 2015 Mar 20;10(3):e0118946. doi: 10.1371/journal.pone.0118946

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© 2015 Yamamoto et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — (A) Immunofluorescence staining of COS1 cells transfected with SNV-harboring CLCN6 variants. Protein disulfide isomerase (PDI) is used as marker of the endoplasmic reticulum (ER). FLAG-tagged CLCN6 is merged with PDI, indicating CLCN6 localization in the ER. (B) Western blotting analysis of cell lysates shows no difference in CLCN6 expression.