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. 2015 Feb 3;4(1):e981483. doi: 10.4161/2162402X.2014.981483

Figure 4.

Figure 4.

Migration capacity of specific NK cell subsets. (A) Scheme of murine transplantation model to study migration capacity. One day after sublethal total body irradiation (TBI), mice received 2×106 freshly isolated bone marrow (BM) and congenic CD45.1+ NK subsets. Purified bulk NK cells (B), MoFlo-sorted Kit+CD27+ (C), KitCD27+ (D), KitCD27+CD11b+ (DP) (E) or CD11b+ (F) NK cell subsets show different behaviors in their migration. Bone marrow, spleen, blood and lung of C57Bl/6 mice were analyzed in week 1 and 2 after transplantation by fluorescence cytometry. Kit CD45.1+ NK are indicated by red, Kit+ cells by black dots. One representative experiment out of 3 is depicted with percentages of either Kit+ (black) and Kit (red) indicated in the plots.