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. 2015 Feb 3;4(1):e984523. doi: 10.4161/2162402X.2014.984523

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© 2015 Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Induction of anti-tumor IgG1 and TUBO tumor growth inhibition. TUBO was inoculated s.c. and permitted to grow for (A) 12 d or (B) 17 d until tumors reached an average of 24 or 60 mm³, respectively. (C/D) Adv alone or PBS control was injected i.t. every other day for a total of three times and tumor growth was monitored (*p < 0.05). Tumor-specific anti-neu antibody was measured in serum (*p < 0.05). (E) IgG subclasses were measured in serum from mice in (A) on day 38 using subclass-specific secondary antibodies (*p < 0.05). (F) Mice treated as in (A) were given a secondary TUBO inoculation on the contralateral flank on day 19 and monitored for an additional 20 d.