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. 2015 Jan 12;11(5):3301–3307. doi: 10.3892/mmr.2015.3182

Figure 1.

Figure 1

Activation of the miR-34a/SIRT1/p53 pathway by the miR-34a mimic and 5-FU. (A) Expression of miR-34a was determined by reverse transcription-quantitative PCR (RT-qPCR). (B) The different components of the miR-34a/SIRT1/p53 pathway were detected by western blotting with GAPDH as the loading control. (C–F) The relative expression of p53, ac-p53, SIRT1 and p21 was quantified from the western blot. (G) A model summarizing the findings from this study combined with previously published data, and describing the 5-FU/p53/p21 interactions. Quantitative data are presented as mean ± SD (n=3), with *p<0.05 and **p<0.01. miR, microRNA; ac, acetylated; 5-FU, 5-fluorouracil; SIRT1, sirtuin 1.