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. 2015 Jan 7;11(5):3259–3268. doi: 10.3892/mmr.2015.3150

Figure 5.

Figure 5

Inhibition of Gli1 reduced the invasive and migratory capacity of TGF-β1-induced NSCLC cells. (A) Cells were seeded 48 h subsequent to si-Gli1, si-VE, or blank transfection into the upper transwell chambers, which were either pre-coated with 20 mg Matrigel (invasion assay), or uncoated (migration assay). Following a 24-h resting period, cells which had migrated (upper panels) or invaded (lower panels) were stained with crystal violet and imaged with a phase contrast microscope (magnification, ×100). Five fields were randomly chosen from each filter, and values representing the mean numbers of cells which had (B) invaded or (C) migrated were obtained. Each experiment was performed in triplicate. (D and E) Transwell Matrigel invasion assay results from TGF-β1-stimulated H460 and SK-MES-1 cells treated with 10 μM GANT 61. All values represent the mean number of cells, error bars represent the standard deviation. NS, not significant; *P<0.05, **P<0.005 and ***P<0.001 vs. control. Gli1, glioma-associated oncogene homolog 1; TGF-β1, transforming growth factor-β1; NSCLC, non-small cell lung cancer; si-Gli1, Gli1 siRNA group; si-VE, nonspecific siRNA group.