Figure 1.
IL-18 enhances mevalonate-derived isoprenoid pyrophosphate-induced upregulation of CD25 expression on Vγ9Vδ2 T cells. Peripheral blood mononuclear cells (PBMCs) at 1.5 × 106/mL were stimulated for 20 h in round-bottom 96-well plate with increasing concentrations of mevalonate-derived isoprenoid pyrophosphates in the absence or presence of 100 ng/mL IL-18 . Cells were stained with fluorophore-conjugated antibodies against CD3, Vδ2 and CD25 (or isotype control). The frequency of CD25+ Vδ2 T cells was assessed via cytofluorimetric analys isusing a FACSCanto II. Data are representative of 2 independent experiments.