Figure 2.

Mevalonate-derived isoprenoid pyrophosphates induce proliferation of Vγ9Vδ2 T cells. T cells were isolated and labeled with 0.5 μM carboxyfluorescein diacetate succinimidyl ester (CFSE). CFSE-labeled T cells (1 × 10⁶ cells/mL) were stimulated with 10 μM mevalonate-derived isoprenoid pyrophosphates and 100 U/mL IL-2 in round-bottom 96 wells for 5 days. After staining for Vδ2 using fluorophore-conjugated anti-TCR Vδ2 antibody, cells were analyzed via flow cytometry. Vδ2⁺ T cells were gated and selectively examined for CFSE dye dilution (stimulated: filled histogram; unstimulated control: open histogram). Data are representative of 3 independent experiments analyzing T cells from 3 different donors.