Figure 3.

Cell-extrinsic geranylgeranyl pyrophosphate (GGPP) can restore Vγ9Vδ2 T cell proliferation during statin-mediated mevalonate pathway inhibition. (A) γδ T cells were isolated and stimulated with 10 μg/ml of an anti-Vγ9 antibody plus 100 U/mL IL-2 inducing cell aggregation (scale bar: 500 μm), γδ T cell receptor (TCR) downregulation and γδ T cell expansion. (B) Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled γδ T cells were stimulated with an anti-Vγ9 antibody plus 100 U/mL IL-2 and proliferation was inhibited by 2 μg/ml simvastatin (statin). Add-back of 10 μg/ml GGPP partially restored proliferation. Cells were counterstained for CD56 and analyzed by flow cytometry. Data are representative of 3 independent experiments with 2 different donors.