Figure 4.

Mevalonate-derived isoprenoid pyrophosphates, IL-18 and N-acetylcysteine (NAC) increased cellular tolerance of Vγ9Vδ2 T cells to H₂O₂-induced oxidative stress. Peripheral blood mononuclear cells (PBMCs) at 1.5 × 10⁶/mL were seeded in round-bottom 96 wells and treated with 10 μM of the various mevalonate-derived isoprenoid pyrophosphates, 100 ng/mL IL-18 or 100 μM N-acetylcysteine (NAC). Cells were challenged with 10 μM H₂O₂ for 1 h followed by the addition of 5 μM CellROX Deep Red Reagent for the detection of reactive oxygen species (ROS). After 30 min, cells were washed and cellular fluorescence was determined by flow cytometry. Data are representative of 4 independent experiments with 2 different donors.