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Detection of TNF inhibitory soluble factors and VEGF in the serum of melanoma patients. Serum from melanoma patients was collected at different time points: baseline, 2 hours, 1 month and 2 months post-treatments. The presence in the serum of chromogranin A (CgA), soluble tumor necrosis factor (sTNFR) was assessed by ELISA. Statistical analysis of differences between means of soluble factors was performed by 2-tailed Student's t-test with significance defined as p < 0.05.
