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. 2014 Dec 17;3(11):e956012. doi: 10.4161/21624011.2014.956012

Figure 1.

Figure 1.

Western blot analysis of the expression of human epidermal growth factor receptors (HER) and insulin-like growth factor 1 receptor (IGF-1R) in various cancer cell lines. Cells were grown in 6 wells plates to 70–80% confluency prior to cell lysis. Cell lysates were solved in SDS-PAGE, transferred to PVDF membranes and commercial rabbit antibodies for HER-1 (epidermal growth factor receptor) (cell signaling), HER-2 (v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2), (cell signaling), HER-3 (v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) (Santa Cruz) and IGF-1R (insulin-like growth factor 1 receptor) (cell signaling) were used to probe for expression of the different receptors. A goat anti-rabbit IgG HRP secondary antibody and ECL reagents (Bio-Rad) were used for detection.