Figure 2.

HER-3 peptide mimics inhibit proliferation of HER-3 positive cancer cell lines. Cancer cells (MDA-MB-468, MCF-7, JIMT-1 and BXPC3) were treated with HER-3 peptide mimics at different concentrations (25–200 μg/mL) for 1 h prior to ligand stimulation with HRG (50ng/mL). After 72 h of incubation in the presence of the peptide mimics, MTT was used to measure cell proliferation. Percent inhibition was calculated by taking absorbance (abs) readings at 570 nm and using the following equation: (abs. untreated-abs. treated)/abs. untreated × 100. An irrelevant (IRR) peptide was used as a negative control. Values represent the mean of at least three independent experiments in triplicate (n = 3) with error bars that indicate SEM.