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. 2015 Mar 9;144(4):549–560. doi: 10.1111/imm.12409

Figure 4.

Figure 4

Telomere length of cytomegalovirus (CMV) pp65/HLA-A2 (NLV)-specific CD8+ T-cell subsets defined by CD45RA and CD27 expression. Representative example of six-colour flow-FISH to determine telomere length within NLV-specific CD45RA/CD27 subsets (a). Live hybridized cells from HLA-matched CMV responders were gated from total lymphocytes before gating of CD8+ tetramer+ cells. Subsequently CD45RA/CD27 subsets were gate from the tetramer+ population and the MFI of telomere probe for each population was determined. Data were acquired on a BD LSR II. Values within histograms represent telomere MFI of the relevant populations. Telomere length of NLV-specific CD45RA/CD27 CD8+ T-cell subsets stratified by age (b: 6 young and 5 old). Telomere MFI was converted first into an MESF value and then kbp using the MESF standard curve. One dot represents one donor, black dots represent young donors and white dots represent old donors. Statistical analysis was performed using the two-tailed Student t-test. All P values were < 0·05.