Figure 4.

Human neutrophil peptide-1 (HNP-1) and human β defensin-1 (HBD-1) increase the activation of CD91^bright monocyte-derived dendritic cell (moDC) subset. Immature moDCs were stimulated with 100 ng/ml recombinant HNP-1, 500 ng/ml recombinant HBD-1, or 100 ng/ml lipopolysaccharide (LPS). After 18 hr, the cells were analysed by flow cytometry to measure cell-surface activation and maturation markers expression on total moDCs (shaded bars), CD91^dim moDCs (white bars) and CD91^bright moDCs (black bars). Graphs show the mean fluorescence intensity (MFI) of CD80, CD86, CD40, CD83 and HLA-DR molecules. Data shown as mean ± SE from six independent experiments and analysed by the paired t-test. ^#P < 0·05 stimulated total moDCs compared to unstimulated (NS) cells; *P < 0·05: CD91^dim moDCs compared with CD91^bright moDCs.