Figure 1.

U2OS cells undergo cell death in response to ADR. (A) Phase-contrast micrograph. U2OS cells were treated with the indicated concentrations of ADR or left untreated. Twenty-four hours after treatment, phase-contrast micrographs were taken. Scale bar = 100 μm. (B) Trypan-blue exclusion assay. U2OS cells were treated as described in (A). Twenty-four hours after treatment, cells were trypsinized and stained with 0.4% of trypan blue. Cells restricting trypan-blue entry were considered viable. (C) Annexin V and PI double staining. U2OS cells were treated with 0.5 μm of ADR (filled boxes) or left untreated (gray boxes). Twenty-four hours after treatment, cells were subjected to annexin V and PI double staining.