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Knockdown of p73 attenuates ADR-mediated induction of p53/TAp73-target gene expression. U2OS cells were transfected with control siRNA or with siRNA against p73. Twenty-four hours after transfection, cells were treated with 0.5 μm of ADR or left untreated. Twenty-four hours after treatment, whole cell lysates and total RNA were extracted and processed for (A) immunoblotting and (B) RT-PCR, respectively.
