Fig 5. The function of hppD in pigmentation in A. media strain WS.
(A) Wild-type A. media strain WS, WSΔhppD, WSΔhppD (pBBR1MCS-5) and WSΔhppD (pBBR1MCS-5-hppD) were cultured in LB, and then at 72 h and 96 h post-inoculation, the OD400 of the cultures were determined. (B) Photographs of 72 h LB cultures of the strains. (C) HPLC chromatograms of culture supernatants of wild-type A. media strain WS, WSΔhppD and WSΔhppD (pBBR1MCS-5-hppD). Samples were taken after 24 h of growth at 30°C in LB. (D) MS analysis of the sample from wild-type A. media strain WS and WSΔhppD (pBBR1MCS-5-hppD), the arrow indicates the molecular ion peaks at 167, the same to that of HGA.