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. Author manuscript; available in PMC: 2015 Sep 19.
Published in final edited form as: Nature. 2014 Dec 22;519(7543):370–373. doi: 10.1038/nature14028

Figure 2. TyrRS facilitates the activation of PARP-1 in an active-site-dependent manner.

Figure 2

a, top, TyrRS activates PARP-1 in an in vitro assay. a, middle. Resveratrol potentiates TyrRS mediated activation of PARP-1. a, bottom. Tyr-SA blocks the resveratrol-mediated activation of PARP-1. b, top. TyrRS-V5 overexpression activates PARP-1 in HeLa cells in a concentration-dependent manner. b, middle. Resveratrol treatment activates PARP-1 in HeLa cells and enhances TyrRS interaction with PARP-1. b, bottom. Tyr-SA blocks the resveratrol-mediated interaction of TyrRS and activation of PARP-1. c, Cartoon illustration of the C-domain disposition in TyrRS (left) and Y341ATyrRS (right). d. Y341ATyrRS enhances its interaction and activates PARP-1 compared to WT.