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. Author manuscript; available in PMC: 2015 Sep 19.
Published in final edited form as: Nature. 2014 Dec 22;519(7543):370–373. doi: 10.1038/nature14028

Extended Data Figure 4. Tyrosyl-AMP analogue (Tyr-SA) does not affect DNA-dependent auto-PARylation of PARP-1.

Extended Data Figure 4

a, Silver stained SDS-PAGE gel showing the purity and input of PARP-1 and TyrRS in the in vitro PARylation study of figure 2. b, Quantitation (Image J software) of the band intensity of PARylated PARP-1 in figure 2a top. c, Tyrosyl-AMP analogue (Tyr-SA) does not affect DNA-dependent auto-PARylation of PARP-1. d, Overexpression of nuclear translocation-weakened mutant of TyrRS6 is less effective in activating PARP-1. e. Y314A-TyrRS is more sensitive to RSV than is TyrRS in facilitating PARP-1 activation.