Extended Data Figure 5. Resveratrol enhances the acetylation of Tip60 and modulates [NAD⁺] in a dose and time dependent manner.

a, Treatment of HeLa cells (1 h) with increasing concentration of resveratrol enhances the acetylation level of Tip60. Activation of Tip60 was monitored by histone acetylation status. b, Total NAD⁺ content of serum starved cells or RSV treated samples were compared with untreated samples at 15 min using a commercially available BioVision NAD⁺/NADH quantitation colorimetric kit. c, Total nicotinamide or ADP-ribose produced was deduced from the difference in the amount of NAD⁺ in each sample with respect to the untreated sample (consumption of one mole of NAD⁺ would give rise one mole of nicotinamide and one mole of ADP-ribose). d, Total NAD⁺ content of the serum starved cells or RSV treated samples were compared with untreated samples at 1 hour. (Although the experiments were done in biological triplicates (all samples showing similar results), the error bars in the figure represent the deviations from the mean of the technical triplicates from one representative biological sample.) e,f Time course study of poly-ADP-ribosylation status and associated signaling events after (e) serum starvation (extended time course data of the same image shown in figure 3c) and (f) treatment with 5 μM RSV. Using the respective antibodies, Activation of p53 was monitored by the induction of p21 and SIRT6. Activation of NRF2 was monitored by HO-1 induction.