Two bouts of rest-inserted loading (d1,8, d1,15, d8,15) were sufficient to demonstrate significant load main effects for p.MAR and p.BFR, but not p.MS (A). There was no significant effect of protocol nor were there any treatment interactions. A comparison of pooled 2, and 3 bouts of loading data compared to 9 bouts of loading demonstrated significant loading and protocol main effects and interaction for p.MAR, p.MS, and p.BFR. Post hoc analysis indicated that 2, 3 and 9 bouts of loading significantly enhanced p.MAR and p.BFR compared to contralateral tibiae, while 3 and 9 bouts of loading were required to significantly enhance p.MS compared to contralateral tibia (*, p<0.05). While 2 bouts of loading generated significantly less p.MAR, p.MS, and p.BFR compared to 3 and 9 bouts of loading (^, p<0.05), no differences were observed between 3 and 9 bouts of loading.