Table 1. Molecular weights of Phamacia standard protein markers with Log10 and Rf values.
Molecular weight(Daltons) | Log10 | Electrophoretic mobility (Rf) |
---|---|---|
14,400 | 4.15836 | 0.96 |
20,100 | 4.30319 | 0.73 |
30,000 | 4.47712 | 0.52 |
43,000 | 4.63346 | 0.29 |
67,000 | 4.82607 | 0.23 |
94,000 | 4.97312 | 0.08 |
Rf values were calculated using the formula; Rf = (distance covered by protein samples) / (distance covered by dye front).