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. 2015 Mar 20;10(3):e0120100. doi: 10.1371/journal.pone.0120100

Fig 4. Polymerisation activity of RDR2-HA and RDR6-HA on partial dsRNA templates.

Fig 4

(A) Purified RDR2-HA and RDR2m-HA were incubated with partial dsRNAs (S3 Fig.) formed with RNA37 hybridized with either 5’[32P]-labeled 21-, 22- or 24-nt ssRNA (RNA21☢, RNA22☢, RNA24☢) (lanes 1–9). Purified RDR2-HA was incubated with either RNA21☢, RNA22☢, RNA24☢ oligonucleotides (lanes 10–12). (B) Purified RDR6-HA and RDR6m-HA were incubated with partial dsRNAs formed with RNA37 hybridized with either RNA21☢, RNA22☢ or RNA24☢ oligonucleotides (lanes 1–9). Purified RDR6-HA was incubated with RNA21☢, RNA22☢, RNA24☢ oligonucleotides (lanes 10–12). (C) Purified RDR2-HA and RDR6-HA were incubated with partial dsRNAs formed with RNA37A hybridized with 5’[32P]-labeled RNA21☢ oligonucleotide and with various concentrations of all four cold NTPs. (*) A single star indicates the position of the 5’[32P]-labeled ssRNA. (**) A double star indicates the position of the full-length elongated RNA template.