Table 1. List of primer sequences used in this study.
| Gene | Primer set | Annealing temperature (°C) | Amplicon size (bp) | GenBank accession number |
|---|---|---|---|---|
| HDAC1 | F: GGAAATCTATCGCCCTCACAR: AAACACCGGACAGTCCTCAC | 60 | 157 | XM_003356305.2 |
| HDAC2 | F: AACCTGCTGCTTGGAGAAAAR: ACCATCAGGATGCAAAGCTC | 60 | 201 | XM_001925318.3 |
| HDAC3 | F: CAACCAGGTGGTGGACTTCTR: GCAGAGGGATGTTGAAGCTC | 60 | 152 | NM_001243827.1 |
| HDAC4 | F: GGTCCTCGCCTACCTTATCCR: GACGCCTGGTAGTTCCTCAG | 60 | 189 | XM_003359701.2 |
| HDAC5 | F: AGATGCACTCCTCCAGTGCTR: GGATGATGGCAAATCCATTC | 60 | 102 | XR_135351.1 |
| HDAC6 | F: ATGGACGGGTATTGCATGTTR: GCGGTGGATGGAGAAATAGA | 60 | 168 | XM_003360315.2 |
| HDAC7 | F: CGTCCCCTACAGAACTCTCGR: TCAGGTTGGGCTCAGAGACT | 60 | 146 | XM_003355640.2 |
| HDAC8 | F: GGTGACGTGTCTGATGTTGGR: AGCTCCCAGCTGTAAGACCA | 60 | 165 | XM_003360365.2 165 |
| HDAC9 | F: AACTGAAGCAACCAGGCAGTR: CCCAACTTGTCCCAGTGAGT | 60 | 149 | XM_003122063.2 |
| HDAC10 | F: TCCATCCGAGTACCTTCCACR: GGCTGCTATGGCCACACTAT | 60 | 179 | XM_003362070.1 |
| HDAC11 | F: GACAAGCGCGTGTACATCATR: AGGTTCCTCTCCACCTTCGT | 60 | 143 | XM_003483230.1 |
| DNMT1 | F: GCGGGACCTACCAAACATR: TTCCACGCAGGAGCAGAC | 60 | 133 | DQ060156 |
| DNMT3a | F: CTGAGAAGCCCAAGGTCAAGR: CAGCAGATGGTGCAGTAGGA | 60 | 238 | NM_001097437 |
| CD40 | F: TGAGAGCCCTGGTGGTTATCR: CTCTCTTTGCCATCCTCCTG | 60 | 235 | NM_214194.1 |
| CD80 | F: TCAGACACCCAGGTACACCAR: GACACATGGCTTCTGCTTGA | 60 | 189 | NM_214087.1 |
| CD86 | F: TTTGGCAGGACCAGGATAACR: GCCCTTGTCCTTGATTTGAA | 60 | 152 | NM_214222.1 |
| TLR4 | F: ATCATCCAGGAAGGTTTCCACR: TGTCCTCCCACTCCAGGTAG | 58 | 235 | NM_001097444.1 |
| MD2 | F: TGCAATTCCTCTGATGCAAGR: CCACCATATTCTCGGCAAAT | 60 | 226 | NM_001104956.1 |
| NF-κB1 | F: TGGGAAAGTCACAGAAACCAR: CCAGCAGCATCTTCACATCT | 60 | 187 | NM_001048232.1 |
| TBP | F: GATGGACGTTCGGTTTAGGR: AGCAGCACAGTACGAGCAA | 60 | 124 | DQ845178.1 |
| IFN-γ | F:AGCTCCCAGAAACTGAACGAR:AGGGTTCAAAGCATGAATGG | 60 | 225 | NM_213948.1 |
| TNF-α | F: CCACCAACGTTTTCCTCACTR: CCAAAATAGACCTGCCCAGA | 60 | 247 | NM_214022.1 |
| IL-1ß | F: GTACATGGTTGCTGCCTGAAR: CTAGTGTGCCATGGTTTCCA | 59 | 137 | NM_001005149.1 |
| IL-8 | F:TAGGACCAGAGCCAGGAAGAR:CAGTGGGGTCCACTCTCAAT | 60 | 174 | NM_213997.1 |
| HPRT1 | F: AACCTTGCTTTCCTTGGTCAR: TCAAGGGCATAGCCTACCAC | 60 | 150 | NM_001032376.2 |
F: Forward primer; R: Reverse primer; bp: base pair.