Table 3.

Demographic expansion tests performed for SAMOVA groups of Ficus insipida subsp. insipida in Amazonia. Time since the expansion was estimated using the mutation rate for the total length of each DNA marker (μ_trnH–psbA = 4.40 × 10⁻¹⁰ s s⁻¹ yr⁻¹ × 340 bp, and μ_ITS = 1.64 × 10⁻⁹ s s⁻¹ yr⁻¹ × 635 bp). Note that P-values for Fu's F_S are only considered significant at the 95% level if the P-value is lower than 0.02 (Excoffier & Lischer, 2010).

DNA marker	SAMOVA groups	Sample size	Neutrality test		Mismatch distribution
			Fu's FS	P-value	τ	P-value	Time [95% CI]
trnH–psbA	V	20	2.51	0.89	6.99	0.16
	VI	24	0.92	0.71	0.00	0.00
	VII	138	−1.90	0.21	0.81	0.29	2.69 Ma [1.85–4.12]
ITS	V	9	0.00	n.a.	0.00	0.00
	VI	10	0.59	0.04	3.00	0.06
	VII	58	−2.21	0.03	3.00	0.14	1.44 Ma [0.23–1.68]

τ, parameter tau of demographic expansion; n.a., not applicable.