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. 2015 Mar 20;10(3):e0121285. doi: 10.1371/journal.pone.0121285

Fig 7. Inhibition of Kv current by KT 5720 is abolished by disruption of caveolae.

Fig 7

(A) Representative Kv currents following 60 min pre-treatment with 2% methyl-β-cyclodextrin (MβCD) before and after application of 1 μM KT 5720. (B) Mean I-V plots (normalized to control current in the presence of MβCD at +60 mV), before and after application of 1 μM KT 5720 (n = 5). (C) Representative Kv currents in the presence of the caveolin scaffolding-domain peptide (CSDP, 100 μM) in the patch pipette, before and after application of KT 5720 (1 μM). (D) Mean I-V plots (normalized to the current at +60 mV in the presence of CSDP) before and after application of 1 μM KT 5720 (n = 5). (E) Representative Kv currents in the presence of a scrambled version of the caveolin scaffolding-domain peptide (CSDP-scr, 100 μM) in the patch pipette before and after application of KT 5720 (1 μM). (F) Mean I-V plots (normalized to the current at +60 mV in the presence of CSDP-scr) before and after application of 1 μM KT 5720 (n = 6, *P<0.05; two-way ANOVA, Bonferroni’s post hoc test).