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. 2015 Mar 20;10(3):e0121082. doi: 10.1371/journal.pone.0121082

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© 2015 Luo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — Pancreatic cancer cell lines were transfected with one of the two HNF1A siRNAs or a control siRNA. The proliferation rate of the siRNA-transfected cells was assessed by CellTiter-Glo assay on AsPC-1 (A) and BxPC-3 (B) cells 12 h to 72h after transfection. Cell cycle distribution was determined by Fluorescence-activated cell sorting analysis in AsPC-1(C) and BxPC-3 (D) cells. Cells were stained with PI, and analyzed for DNA content using flow cytometry 72h following transfections. The populations of cells in G1, S, and G2 phases are characterized by different intensities of PI-A fluorescence and are indicated. Data are shown relative to the control. *P<0.05 compared with each corresponding control.