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. 2015 Mar 20;10(3):e0120662. doi: 10.1371/journal.pone.0120662

Fig 3. hth mutant nuclei show increased number of DNA breaks.

Fig 3

A,A´) Wild type syncytium (cycle 11) stained for His2AvP (green) and topro3 (blue). Due to the lack of mitotic checkpoints during the rapid syncytial divisions the nuclei show some degree of DNA breaks marked with the anti-His2AvP antibody (arrowheads in A´). B, B´) However, hth mutant nuclei show more nuclear signal when stained with the same antibody (see arrows in B´) indicating that they have more breaks in their DNA. C) Quantification of the DNA breaks in the Dfhth mutant embryos and in their wild type siblings. Quantification was performed by counting the number of nuclear dots marked with the anti-His2AvP antibody. (N = 50 nuclei for each genotype from 5 different embryos each. P-value: 7,0085X10−5).