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. 2015 Mar 20;10(3):e0120662. doi: 10.1371/journal.pone.0120662

Fig 5. The nuclei of hth mutants have dispersed rDNA foci and ectopic nucleoli and produce more rRNA.

Fig 5

A) Wild type nuclei stained with anti-Fibrillarin (red) and a specific probe that recognizes the rDNA genomic region (green). B) hth mutant nuclei stained as in A. The mutant nuclei exhibit an increased number of nucleoli with different sizes. C) Quantification of nuclear dots marked with both the rDNA probe and anti-Fibrillarin in wild type and mutant nuclei. 45 nuclei were quantified for each genotype. (p-value: 0,00010132, calculated using T-Test). D) qRT-PCR comparing the levels of 18S RNA transcripts in wild type and Dfhth mutant embryos. The experiment was performed three independent times to be significant (p-value: 0,01046, calculated using T-Test). Error bars in the graph show the standard deviation and have being calculated as in Fig. 1E. The experiment was normalized using the bicoid (bcd) mRNA.