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. 2015 Mar 20;10(3):e0120627. doi: 10.1371/journal.pone.0120627

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© 2015 Zhang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Cytorrhysis assay using glycerol was performed to compare the appressorial turgor pressure of MoSFA1 mutant and wild-type strain. Different glycerol solutions were given to appressoria at 48 hpi. Appressorial cytorrhysis was counted under an optical microscope. The rate of cytorrhysis was the average of three replications. (B) Infection rate of M. oryzae strains in barley leaf cells at 36 hpi. (C) Growth of infectious hyphae of MoSFA1 deletion mutant was retarded in barley leaf cells. Penetration and infectious hyphae were examined under optical microscopy. Ap, appressorium; PI, primary infectious hyphae; IH, secondary infectious hyphae. Significant difference analysis was performed after percentages were arcsine transformed. The original percentages were used for presentations. Error bars represent SD. Asterisks in each data column indicate significant differences at p = 0.05.