Fig 4. Decreased naive CD4 and CD8 T cells with increased apoptosis in Rasal3-KO mice.

(A) Flow cytometry profiles for CD4 and CD8 of splenocytes from WT and Rasal3-KO mice (left). The bar graphs show the number of total splenocytes and number and frequency of splenic CD4⁺ TCRβ⁺ and CD8⁺ TCRβ⁺ cells (n = 19 mean ± s.e.) (right). (B and C) Profiles of CD44 and CD62L in splenic CD4⁺ TCRβ⁺ (B) or CD8⁺ TCRβ⁺ (C) cells (left). The bar graphs show the number and frequency of naive (CD44^lo CD62L^hi) cells, effector memory (CD44^hi CD62L^lo) cells and central memory (CD44^hi CD62L^hi) cells (n = 17, mean ± s.e.) (right). (D) Annexin V and 7AAD staining of CD4⁺ TCRβ⁺ CD44^lo, CD8⁺ TCRβ⁺ CD44^lo and CD4^- CD8^- TCRβ^- spleen cells freshly isolated from WT or Rasal3-KO mice. (E) The bar graphs show the frequency of Annexin V⁺ 7AAD^- and Annexin V⁺ 7AAD⁺ cells depicted in panel D (n = 5, mean ± s.e.).