Fig 4. Analysis of molecular mediators in divergent Low and High CORT groups.
Effect of the aggrupation dependent on the basal levels of CORT on the expression of two pro-inflammatory (IFN-γ and IL-1β, panels A and B) and two anti-inflammatory mediators (IL-13 and 4, panels C and D). Data are adjusted means (number inside bars) ± SE. Number of birds per group = 10. Different letters indicate significant (p < 0.05) differences between groups. The relative expression of each target gene was calculated using the formula: Gene Level = 2-(Target Gene Ct – β-Actin Ct). The value obtained was then multiplied by 1x108 in order to fit the scale of the graphs.