Table 2. Characteristics of the microsatellite loci, including the type of fluorescent dye (Dye) and the mixing ratio of fluorescent and non-fluorescent forward primers (Fl: Non-fl), the observed number of alleles (N _A), observed (H _O) and expected (H _E) heterozygosity, probability of exclusion when one parent is known (P _EX), and estimated null allele frequency by locus for the 60 individual Castanea crenata trees at the Field Research Center, Tohoku University, Miyagi, Japan.

Locus	Dye	Fl : Non-fl	NA	HO	HE	PEX	Null allele frequency	Reference
CsCAT2	VIC	2 : 8	6	0.450	0.415	0.247	− 0.0571	Marinoni et al. [65]
CsCAT5	PET	10 : 0	13	0.717	0.681	0.459	+ 0.0334	Marinoni et al. [65]
CsCAT14	6-FAM	1 : 9	6	0.733	0.669	0.415	− 0.0502	Marinoni et al. [65]
EMCs2	PET	1 : 9	3	0.617	0.588	0.031	− 0.0223	Buck et al. [66]
EMCs17	PET	2 : 8	2	0.383	0.333	0.138	− 0.0750	Buck et al. [66]
KT001b	6-FAM	2 : 8	12	0.683	0.675	0.430	− 0.0061	Yamamoto et al. [67]
KT004a	VIC	4 : 6	12	0.750	0.790	0.594	+ 0.0254	Yamamoto et al. [67]
KT005a	NED	8 : 2	13	0.917	0.859	0.708	− 0.0369	Yamamoto et al. [67]
KT020a	NED	2 : 8	5	0.633	0.535	0.324	− 0.1258	Yamamoto et al. [67]
KT024a	6-FAM	1 : 9	4	0.317	0.296	0.157	− 0.0382	Yamamoto et al. [67]
KT030a	NED	3 : 7	7	0.867	0.757	0.537	− 0.0726	T. Yamamoto, pers. comm.
Mean			7.5	0.642	0.600	0.997*

These parameters were calculated using CERVUS 3.0.3 [54].

*Overall combined exclusion probability