Fig 8. Effect of sesamin on Ang II induced Smad3 phosphorylation.

Rat cardiac fibroblasts were isolated from 0 to 3-day-old neonatal rats. Following incubation with sesamin (1.0, 10.0 and 100.0 μmol/L) for 12 h, cells were exposed to Ang II (0.1μmol/L) for 24 h. Cell lysates were subjected to western blot analysis for Smad3 phosphorylation as detailed in the Methods section. Levels of p-Smad3 protein were normalized to that of total Smad3 protein. Data were presented as percentage of that in control group (mean ± SD, n = 3). a P <0.05, b P <0.01 vs. control group; c P <0.05, d P <0.01 vs. Ang II group.