Table 2. Matrix Effect of QC Samples for Exe, 17DhExe and Exe17Oglu and their internal standards.
| Analyte | Nominal Conc. (ng/mL) | *Matrix Effect (%) | *Matrix Effect of IS at 10 ng/mL (%) | # Relative Matrix Effect (%) |
|---|---|---|---|---|
| Exe | 3.0 | 64.2 ± 11.8 | 62.2 ± 7.18 | 103.2 |
| 7.5 | 62.3 ± 7.65 | 64.3 ± 9.03 | 96.9 | |
| 25.0 | 61.4 ± 10.6 | 60.1 ± 4.35 | 102.2 | |
| 17DhExe | 1.5 | 53.3 ± 5.31 | 54.4 ± 9.83 | 98.0 |
| 4.0 | 52.1 ± 3.13 | 53.0 ±8.76 | 98.3 | |
| 10.0 | 53.4 ± 4.41 | 55.3 ± 6.54 | 96.6 | |
| Exe17Oglu | 1.5 | 33.1 ± 4.88 | 31.2 ± 5.02 | 106.1 |
| 4.0 | 34.1 ± 3.16 | 35.0 ± 3.69 | 97.4 | |
| 10.0 | 35.3 ± 2.65 | 35.2 ± 2.84 | 100.3 |
*Expressed as average percentage (n = 4) of peak area of analyte in matrix-based tube with that in reference tube
#Expressed as ratio of matrix effect on compound to that of IS