Fig 3. Insulin signaling and fatty acid partitioning in human primary skeletal muscle cells.
Muscle cells were incubated with vehicle (CON; cross-hatched), PALM (open), NORM (filled), or HSFA (hatched). (A) Delta pAktThr308/Akt (calculated as the difference between insulin-stimulated and basal pAktThr308/Akt), and non-insulin-stimulated (i.e., basal) accumulation of (B) DAG and (C) TAG. Cells were exposed for 12h to a no fatty acid control condition (CON) or 0.4mM of one of the 3 different fatty acid treatments (PALM, NORM, and HSFA). For all panels, data are expressed relative to CON. *P<0.05 vs. CON. DAG, diacylglycerol; TAG, triacylglycerol; AU, arbitrary units.