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. 2015 Mar 20;10(3):e0119402. doi: 10.1371/journal.pone.0119402

Fig 4. α-Catulin knockdown enhances susceptibility of melanoma cells to cisplatin.

Fig 4

(A-F) Stable infected (n.s., sh-catu2) (A) Mel.7, (B) Mel.17, (C) Mel.15, (D) Mel.7 spheroids, (E) Mel.7 (n.s., sh-catu1) or (F) Melanocytes (mock, myc-α-catulin) were treated with different concentrations of cisplatin for 48h and cell survival normalized to untreated cells (pos. contr.). Viability was analyzed by CellTiter-Blue Assay. (G) Stable infected Mel.7 spheroids were treated with 200 μg/ml cisplatin for 96 hours and diameter of the spheroids determined before and after treatment and statistically evaluated. Observations (•) mean (-) n = 14, (H) Microscopic images from Mel.7 spheroids.