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. 2015 Mar 20;11(3):e1005083. doi: 10.1371/journal.pgen.1005083

Fig 3. Global reduction of H3K9me3 in dim-3compromises telomeric silencing.

Fig 3

[A] Western blots of histones isolated from indicated strains were probed for H3K9me3, or histone H3 (hH3) as a loading control. [B] (Left) Western blots of histones isolated from the indicated strains grown with or without histidine probed for H3K9me3 or hH3. (Right) Quantification of H3K9me3 levels from three independent experiments, normalized to hH3 levels. [C] Chromatin Immunoprecipitation sequencing (ChIP-seq) of H3K9me3 from dim-3 + (WT) [56] and dim-3 strains displayed on IGV, as in Fig. 1B. Due to the vastly reduced trimethylation of H3K9 in the dim-3 strain, this strain shows a reduced signal-to-noise ratio, rendering background more prominent. [D] Growth of strains containing the telVR::hph or cenVIR::bar reporter cassettes on minimum medium (MIN) plates or plates supplemented with hygromycin (200 μg/mL, +HYG, left) or phosphinothricin (8 mg/mL, +BASTA, right). Approximate numbers of spotted conidia indicated below the pictures.