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. 2015 Mar 20;10(3):e0119872. doi: 10.1371/journal.pone.0119872

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© 2015 Akil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — (A-C) Quantitative RT-PCR analysis of IL-22R (IL-22R1, IL-10R2) and IL-22 in total RNA extracted from 10 GBM-initiating cells established from GBM tumors (GL). Three independent NHEK cultures were used as a positive control for IL-22R1 (A) and IL-10R2 (B) mRNA expression and three human psoriatic skin biopsies (PSO) were used as a positive control for IL-22 (C). Target gene expression was normalized to the housekeeping GAPDH mRNA. (D) Detection of IL-22R1 and IL-10R2 proteins assessed by western blot analysis from two GBM-initiating cells. Positive control was HT29 cell line. Actin was used as a loading protein control.