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. 2015 Jan 29;18(2):pyu072. doi: 10.1093/ijnp/pyu072

Figure 6.

Figure 6.

Chronic intermittent ethanol (CIE) induced DNA demethylation and histone H3K9 acetylation in the NR2B promoter in the prefrontal cortex of the mice. After 5 days withdrawal from CIE exposure, 8 mice from each group were sacrificed and the tissues of prefrontal cortex were collected. (A) Genomic DNA isolated from these mice was subjected to bisulfite pyrosequencing analysis. The values in negative are defined in this study to represent demethylation. Results are presented as the mean percentage of demethylation level in the CIE group vs control group±SEM; *P<.05; **, P<.01 compared with their corresponding controls. (B) Eight mice from each group were sacrificed and prefrontal cortexes were collected. Quantitative chromatin immunoprecipitation (qChIP) assays were performed using these prefrontal cortical tissues to examine the acetylation levels of histone H3K9 in the NR2B promoter in the regions b, c, and g of the NR2B (Qiang et al., 2010). Values are presented as average fold changes over their corresponding controls (control=1)±SEM (n=8). *P<.05, **P<.01 compared with their corresponding controls (2-way analysis of variance [ANOVA] followed by posthoc Newman-Keuls test).