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Chronic intermittent ethanol (CIE) and Trichostatin A (TSA) increase NR2B gene transcription. Eight mice in each treatment group of CIE, air, or TSA were sacrificed, and total RNA was isolated from the prefrontal cortex using the mirVanaTM miRNA isolation kit (Ambion, AM1561). Two-step quantitative PCR (qPCR) was performed as previously described (Qiang et al., 2011). Results are presented as the mean ratio to control ± SEM; **P<.01 compared with control levels.
