Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Thorax. 2013 Nov 20;69(4):335–345. doi: 10.1136/thoraxjnl-2013-204324

Figure 4.

Figure 4

Effector memory phenotype of Phl p 5 peptide 26 (p26) tetramer-positive CD4 T cells from subjects with allergy. Peripheral blood mononuclear cells isolated from subjects with allergy were labelled immediately ex vivo with Phl p 5b peptide 26 tetramer. This was followed by cell surface staining with FL-1 conjugated cell surface maker antibodies and an anti-CD4PE-Cy5. The expression of a FL-1 conjugated cell surface maker antibody was compared on total CD4high and CD4 human leukocyte antigen (HLA)-DR1 Phl p 5 p26 tetramer-positive cells from HLA-DR1-positive individuals with grass allergy. The reduction in mean fluorescence intensity for CD62L and CD45RA staining of tetramer-positive cells is indicated in online supplementary figure S1. Statistical analysis was performed using a Wilcoxon signed rank test. Samples were processed during the grass pollen season, summer 2010.