Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Thorax. 2013 Nov 20;69(4):335–345. doi: 10.1136/thoraxjnl-2013-204324

Table 3.

Sequence alignment of the in silico defined epitope sequence (YAVFEAALT) within Phl p 5b peptide 26 (p26), from Phleum pratense and other commonly occurring grass species allergens in the UK

Grass allergen p26 epitope sequence Percentage sequence alignment
Phl p 5.0201 YAVFEAALT –
Dac g 5 isoallergen 2 YAVFEAALT 100
Dac g 5 isoallergen 1 YAVFEAALT 100
Dac g 5 YAVFEAALT 100
Lol p VA YAVFEAALT 100
Lol p 5C YAVFEAALT 100
Hol l 5.0201 YAVFEAALT 100
Hol l 5b FAVFKAALT 77
Poa p 5.0201 FAVFKAALT 77
Phl p 5.0101 YTVFETALK 66
Hol l 5.0101 YTVFETALK 66
Lol p 5.0101 YTVSETALK 55

The TEPITOPE-predicted HLA-DR1-restricted epitope within Phl p 5b-derived p26 (YAVFEAALT) was identified in other grass species. Sequences were aligned using the CLUSTAL 2.0.12 multiple sequence alignment programme available via the European Bioinformatics Institute server: (http://www.ebi.ac.uk/Tools/services/web/toolform.ebi?tool=clustalw2). The Phl p isoallergen investigated in this study is shown in bold. The table highlights the percentage identity of the epitope sequences following calculation of pairwise sequence alignment scores when compared with Phl p 5.0201. Dac g, Dactylis glomerata; HLA, human leukocyte antigen; Hol l, Holcus lanatus; Lol p, Lolium perenne; Phl p, Phleum pratense; Poa p, Poa pratensis.