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. 2015 Mar 12;8:154. doi: 10.1186/s13071-015-0770-0

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© Melo et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DNA detection in serum, blood and T. cruzi samples by Real Time qPCR. Whole blood was spiked with Exo-IPC (1X) or T. cruzi cells (1 par. eq./ mL) before serum preparation. Results are expressed as the media of Threshold cycles (Ct) and represent the average from five replicate samples ± Standard deviation. *, ** p < 0.05.