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Method for obtaining pure SQCLC tissues for detection ofEGFRgene mutation. [A] HE stained slice diagnosed as SQCLC. [B] TTF-1 staining of the residual alveolar epithelium. When obtaining the SQCLC tissues for mutation analysis, the positive regions (indicated by green borders) were avoided. [C] ∆NP63 staining. The region outlined by the green border was used to detect the EGFR gene mutation status.
