Fig. 2.

Transactivation ability of the wild-type and mutant RUNX2 proteins. COS7 cells were transfected with p6OSE2-luc as a reporter plasmid, full-length, wild-type or mutant RUNX2 as effector plasmids, and phRL-TK as an internal control of transfection efficiency. Data are presented as fold activation relative to the activity obtained with wild-type RUNX2 vector plasmid. Bars represent the average ratios of luciferase to Renilla activity. Standard deviations are represented by error bars. Both the 27Q variant and ΔTLT198_200 mutants showed significantly reduced transactivation ability compared to the wild type. Moreover, transactivation of the 27Q variant was significantly lower than that of the ΔTLT198_200 mutant.