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Time course of TNF-α stimulation of MCP-1 mRNA expression in cultured podocytes. Differentiated mouse podocytes were treated with 10 ng/ml recombinant mouse TNF-α for the indicated periods of time up to 48 h. TNF-α potently increased MCP-1 gene expression, assayed by RT-qPCR, in treated podocytes at 24 h (13.3-fold) and 48 h (11-fold). *** p < 0.005 vs. control (n = 3).
