(A) HCC2279 and H1975 cells were serum-starved overnight and then either treated with vehicle or dasatinib (30 nM) for 8 hours. Cells were harvested and Fn14, p-Src, Src, and GAPDH levels were analyzed by Western blotting. (B) A549 cells were serum-starved overnight and then either left untreated or treated with EGF (50 ng/mL) alone, EGF and drug vehicle, or EGF and dasatinib (30 nM) for the indicated time periods. Cells were harvested and Fn14, p-Src, Src, p-EGFR, EGFR, and GAPDH levels were analyzed by Western blotting.